Rosenmund Lab

Control of neurotransmitter release by two distinctmembrane-binding faces of the Munc13-1 C₁C₂B region.
Camacho M, Quade B, Trimbuch T, Xu J, Sari L, Rizo J, Rosenmund C.
Elife. 2021 Nov 15;10:e72030. doi: 10.7554/eLife.72030.

Reexamination of N-terminal domains of Syntaxin-1 in vesicle fusion from central murine synapses.
Vardar G, Salazar-Lázaro A, Brockmann MM, Weber-Boyvat M, Zobel S, Kumbol VW, Trimbuch T, Rosenmund C.
Elife. 2021 Aug 24;10:e69498. doi: 10.7554/eLife.69498.

Impaired inhibitory GABAergic synaptic transmission and transcription studied in single neurons by Patch-seq in Huntington's disease.
Paraskevopoulou F, Parvizi P, Senger G, Tuncbag N, Rosenmund C, Yildirim F.
Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2020293118. doi: 10.1073/pnas.2020293118.

Cannabinoid receptor activation acutely increases synaptic vesicle numbers by activating synapsins in human synapses.
Patzke C, Dai J, Brockmann MM, Sun Z, Fenske P, Rosenmund C, Südhof TC.
Mol Psychiatry. 2021 Apr 30. doi: 10.1038/s41380-021-01095-0.

Biallelic variants in TSPOAP1, encoding the active-zone protein RIMBP1, cause autosomal recessive dystonia.
Mencacci NE, Brockmann MM, Dai J, Pajusalu S, Atasu B, Campos J, Pino G, Gonzalez-Latapi P, Patzke C, Schwake M, Tucci A, Pittman A, Simon-Sanchez J, Carvill GL, Balint B, Wiethoff S, Warner TT, Papandreou A, Soo A, Rein R, Kadastik-Eerme L, Puusepp S, Reinson K, Tomberg T, Hanagasi H, Gasser T, Bhatia KP, Kurian MA, Lohmann E, Õunap K, Rosenmund C, Südhof TC, Wood NW, Krainc D, Acuna C.
J Clin Invest. 2021 Apr 1;131(7):e140625. doi: 10.1172/JCI140625.

Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
J Neurosci. 2020 Dec 2;40(49):9372-9385. doi: 10.1523/JNEUROSCI.1922-20.2020.

SynaptoPAC, an Optogenetic Tool for Induction of Presynaptic Plasticity.
Oldani S, Moreno-Velasquez L, Faiss L, Stumpf A, Rosenmund C, Schmitz D, Rost BR.
J Neurochem. 2020 Oct 9. doi: 10.1111/jnc.15210.

VGLUT2 EXPRESSION IN DOPAMINE NEURONS CONTRIBUTES TO POST-LESIONAL STRIATAL REINNERVATION.
Kouwenhoven WM, Fortin G, Penttinen AM, Florence C, Delignat-Lavaud B, Bourque MJ, Trimbuch T, Luppi MP, Salvail-Lacoste A, Legault P, Poulin JF, Rosenmund C, Awatramani R, Trudeau LÉ
J Neurosci. 2020 Sep 14:JN-RM-0823-20. doi: 10.1523/JNEUROSCI.0823-20.2020.

A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release.
Brockmann MM, Zarebidaki F, Camacho M, Grauel MK, Trimbuch T, Südhof TC, Rosenmund C.
Cell Rep. 2020 Aug 4;32(5):107960. doi: 10.1016/j.celrep.2020.107960.

ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs.
Weber-Boyvat M, Trimbuch T, Shah S, Jäntti J, Olkkonen VM, Rosenmund C.
Cell Mol Life Sci. 2020 Jul 30. doi: 10.1007/s00018-020-03604-w.

Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25.
Malsam J, Bärfuss S, Trimbuch T, Zarebidaki F, Sonnen AF, Wild K, Scheutzow A, Rohland L, Mayer MP, Sinning I, Briggs JAG, Rosenmund C, Söllner TH.
Cell Rep. 2020 Jul 21;32(3):107926. doi: 10.1016/j.celrep.2020.107926.

Epilepsy-causing STX1B mutations translate altered protein functions into distinct phenotypes in mouse neurons.
Vardar G, Gerth F, Schmitt XJ, Rautenstrauch P, Trimbuch T, Schubert J, Lerche H, Rosenmund C, Freund C.
Brain. 2020 Jul 1;143(7):2119-2138. doi: 10.1093/brain/awaa151.

Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice.
Hoffmann-Conaway S, Brockmann MM, Schneider K, Annamneedi A, Rahman KA, Bruns C, Textoris-Taube K, Trimbuch T, Smalla KH, Rosenmund C, Gundelfinger ED, Garner CC, Montenegro-Venegas C.
Elife. 2020 May 4;9:e56590. doi: 10.7554/eLife.56590.

Layer 6b Is Driven by Intracortical Long-Range Projection Neurons.
Zolnik TA, Ledderose J, Toumazou M, Trimbuch T, Oram T, Rosenmund C, Eickholt BJ, Sachdev RNS, Larkum ME.
Cell Rep. 2020 Mar 10;30(10):3492-3505.e5. doi: 10.1016/j.celrep.2020.02.044.

CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A.
Cell Rep. 2020 Feb 18;30(7):2444-2459.e7. doi: 10.1016/j.celrep.2020.01.079.

Calcium-Independent Exo-endocytosis Coupling at Small Central Synapses.
Orlando M, Schmitz D, Rosenmund C, Herman MA.
Cell Rep. 2019 Dec 17;29(12):3767-3774.e3. doi: 10.1016/j.celrep.2019.11.060.

Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses.
Patzke C, Brockmann MM, Dai J, Gan KJ, Grauel MK, Fenske P, Liu Y, Acuna C, Rosenmund C, Südhof TC.
Cell. 2019 Oct 3;179(2):498-513.e22. doi: 10.1016/j.cell.2019.09.011.

The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches.
Brosig A, Fuchs J, Ipek F, Kroon C, Schrötter S, Vadhvani M, Polyzou A, Ledderose J, van Diepen M, Holzhütter HG, Trimbuch T, Gimber N, Schmoranzer J, Lieberam I, Rosenmund C, Spahn C, Scheerer P, Szczepek M, Leondaritis G, Eickholt BJ.
Cell Rep. 2019 Nov 12;29(7):2028-2040.e8. doi: 10.1016/j.celrep.2019.10.039.

LSP5-2157 a new inhibitor of vesicular glutamate transporters.
Poirel O, Mamer LE, Herman MA, Arnulf-Kempcke M, Kervern M, Potier B, Miot S, Wang J, Favre-Besse FC, Brabet I, Laras Y, Bertrand HO, Acher F, Pin JP, Puel JL, Giros B, Epelbaum J, Rosenmund C, Dutar P, Daumas S, El Mestikawy S, Pietrancosta N.
Neuropharmacology. 2020 Mar 1;164:107902. doi: 10.1016/j.neuropharm.2019.107902. Epub 2019 Dec 4.

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, Sigrist SJ, Rosenmund C, Schmitz D.
Elife. 2019 Sep 19;8. pii: e43243. doi: 10.7554/eLife.43243.

Altered inhibition and excitation in neocortical circuits in congenital microcephaly.
Zaqout S, Blaesius K, Wu YJ, Ott S, Kraemer N, Becker LL, Rosário M, Rosenmund C, Strauss U, Kaindl AM.
Neurobiol Dis. 2019 May 15. pii: S0969-9961(19)30122-6. doi: 10.1016/j.nbd.2019.05.008.

Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC.
Elife. 2019 May 10;8. pii: e46629. doi: 10.7554/eLife.46629.

Glutamatergic innervation onto striatal neurons potentiates GABAergic synaptic output.
Paraskevopoulou F, Herman MA, Rosenmund C
J Neurosci. 2019 Apr 1. pii: 2630-18. doi: 10.1523/JNEUROSCI.2630-18.2019.

Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Sci Rep. 2019 Mar 20;9(1):4890. doi: 10.1038/s41598-019-41259-1.

Membrane bridging by Munc13-1 is crucial for neurotransmitter release.
Quade B, Camacho M, Zhao X, Orlando M, Trimbuch T, Xu J, Li W, Nicastro D, Rosenmund C, Rizo J
Elife. 2019 Feb 28;8. pii: e42806. doi: 10.7554/eLife.42806.

Light-activated ROS production induces synaptic autophagy.
Hoffmann S, Orlando M, Andrzejak E, Bruns C, Trimbuch T, Rosenmund C, Garner CC, Ackermann F
J Neurosci. 2019 Jan 17. pii: 1317-18. doi: 10.1523/JNEUROSCI.1317-18.2019.

Differential pH Dynamics in Synaptic Vesicles From Intact Glutamatergic and GABAergic Synapses.
Herman MA, Trimbuch T, Christian Rosenmund
Front Synaptic Neurosci. 2018 Dec 3;10:44. doi: 10.3389/fnsyn.2018.00044. eCollection 2018.

Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis
Shigeki Watanabe, Lauren Elizabeth Mamer, Sumana Raychaudhuri, Delgermaa Luvsanjav, Julia Eisen, Thorsten Trimbuch, Berit Söhl-Kielczynski, Pascal Fenske, Ira Milosevic, Christian Rosenmund, Erik M. Jorgensen
Neuron 98, 1184–1197, June 27, 2018, doi: 10.1016/j.neuron.2018.06.005.

Synaptotagmin-1 drives synchronous Ca²⁺-triggered fusion by C₂B-domain-mediated synaptic-vesicle-membrane attachment.
Chang S, Trimbuch T, Rosenmund C.
Nat Neurosci. 2018 Jan;21(1):33-40. doi: 10.1038/s41593-017-0037-5.

Cooperative binding mitigates the high-dose hook effect.
Roy RD, Rosenmund C, Stefan MI.
BMC Syst Biol. 2017 Aug 14;11(1):74. doi: 10.1186/s12918-017-0447-8.

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.
Piwecka M, Glažar P, Hernandez-Miranda LR, Memczak S, Wolf SA, Rybak-Wolf A, Filipchyk A, Klironomos F, Cerda Jara CA, Fenske P, Trimbuch T, Zywitza V, Plass M, Schreyer L, Ayoub S, Kocks C, Kühn R, Rosenmund C, Birchmeier C, Rajewsky N.
Science. 2017 Aug 10. pii: eaam8526. doi: 10.1126/science.aam8526.

Heterodimerization of Munc13 C2A domain with RIM regulates synaptic vesicle docking and priming.
Camacho M, Basu J, Trimbuch T, Chang S, Pulido-Lozano C, Chang SS, Duluvova I, Abo-Rady M, Rizo J, Rosenmund C.
Nat Commun. 2017 May 10;8:15293. doi: 10.1038/ncomms15293.

Characterization of a Human Point Mutation of VGLUT3 (p.A211V) in the Rodent Brain Suggests a Nonuniform Distribution of the Transporter in Synaptic Vesicles.
Ramet L, Zimmermann J, Bersot T, Poirel O, De Gois S, Silm K, Sakae DY, Mansouri-Guilani N, Bourque MJ, Trudeau LE, Pietrancosta N, Daumas S, Bernard V, Rosenmund C, El Mestikawy S.
J Neurosci. 2017 Apr 12;37(15):4181-4199. doi: 10.1523/JNEUROSCI.0282-16.2017. Epub 2017 Mar 17.

ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1143-1161. doi: 10.1083/jcb.201606086. Epub 2017 Mar 6. Erratum in: J Cell Biol. 2017

Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1C2BMUN.
Xu J, Camacho M, Xu Y, Esser V, Liu X, Trimbuch T, Pan YZ, Ma C, Tomchick DR, Rosenmund C, Rizo J.
Elife. 2017 Feb 8;6. pii: e22567. doi: 10.7554/eLife.22567.

Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons.
Sampathkumar C, Wu YJ, Vadhvani M, Trimbuch T, Eickholt B, Rosenmund C.
Elife. 2016 Oct 26;5. pii: e19374. doi: 10.7554/eLife.19374.

RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, Rost BR, Eickholt BJ, Haucke V, Schmitz D, Sigrist SJ, Rosenmund C.
Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11615-11620. Epub 2016 Sep 26.

Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons.
Vardar G, Chang S, Arancillo M, Wu YJ, Trimbuch T, Rosenmund C.
J Neurosci. 2016 Jul 27;36(30):7911-24. doi: 10.1523

Functional Synergy between the Munc13 C-terminal C1 and C2 domains.
Liu X, Seven AB, Camacho M, Esser V, Xu J, Trimbuch T, Quade B, Su L, Ma C, Rosenmund C, Rizo J.
eLife 2016;10.7554/eLife.13696

Should I stop or should I go? The role of complexin in neurotransmitter release.
Trimbuch T, Rosenmund C.
Nature Reviews Neuroscience  17, 118–125 (2016)

Optogenetic acidification of synaptic vesicles and lysosomes.
Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C.
Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.

Co-release of glutamate and GABA from single vesicles in GABAergic neurons exogenously expressing VGLUT3.
Zimmermann J, Herman MA, Rosenmund C.
Front Synaptic Neurosci. 2015 Sep 23;7:16

Syntaxin 1B is important for mouse postnatal survival and proper synaptic function at the mouse neuromuscular junctions.
Wu Y-J, Tejero R, Arancillo M, Vardar G, Korotkova T, Kintscher M, Schmitz D, Ponomarenko A, Tabares L, Rosenmund C.
Journal of Neurophysiology Published 1 October 2015 Vol. 114 no. 4, 2404-2417 DOI: 10.1152/jn.00577.2015

Ligand-dependent opening of the multiple AMPA receptor conductance states: a concerted model.
Dutta-Roy R, Rosenmund C, Edelstein SJ, Le Novère N.
PLoS One. 2015 Jan 28;10(1):e0116616. doi: 10.1371/journal.pone.0116616.

On the Brink: A New Synaptic Vesicle Release Model at the Calyx of Held.
Herman MA, Rosenmund C.
Neuron. 2015 Jan 7;85(1):6–8

Clathrin regenerates synaptic vesicles from endosomes.
Watanabe S, Trimbuch T, Camacho-Pérez M, Rost BR, Brokowski B, Söhl-Kielczynski B, Felies A, Davis MW, Rosenmund C, Jorgensen EM.
Nature. 2014 Nov 13;515(7526):228-33

The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones.
Imig C, Min SW, Krinner S, Arancillo M, Rosenmund C, Südhof TC, Rhee J, Brose N, Cooper BH.
Neuron. 2014 Oct 22;84(2):416-31

Synaptobrevin 1 mediates vesicle priming and evoked release in a subpopulation of hippocampal neurons.
Zimmermann J, Trimbuch T, Rosenmund C.
J Neurophysiol. 2014 Sep 15;112(6):1559-65.

Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture.
Herman MA, Ackermann F, Trimbuch T, Rosenmund C.
J Neurosci. 2014 Aug 27;34(35):11781-91.

Biophysical properties of presynaptic short-term plasticity in hippocampal neurons: insights from electrophysiology, imaging and mechanistic models.
Dutta Roy R, Stefan MI, Rosenmund C.
Front Cell Neurosci. 2014 May 22;8:141

Re-examining how complexin inhibits neurotransmitter release: SNARE complex insertion or electrostatic hindrance?
Trimbuch T, Xu J, Flaherty D, Tomchick DR, Rizo J, Rosenmund C.
eLife 2014;10.7554/eLife.02391

Biophysical properties of presynaptic short-term plasticity in hippocampal neurons: insights from electrophysiology, imaging and mechanistic models.
Dutta Roy R, Stefan MI, and Rosenmund C.
Front. Cell. Neurosci. | doi: 10.3389/fncel.2014.00141 

Investigation of Synapse Formation and Function in a Glutamatergic-GABAergic Two-Neuron Microcircuit.
Chang CL, Trimbuch T, Chao HT, Jordan JC, Herman MA, Rosenmund C.
J Neurosci. 2014 Jan 15;34(3):855-68.

Nanometer-resolution fluorescence electron microscopy (nano-EM) in cultured cells.
Watanabe S, Lehmann M, Hujber E, Fetter RD, Richards J, Söhl-Kielczynski B, Felies A, Rosenmund C, Schmoranzer J, Jorgensen EM.
Methods Mol Biol. 2014;1117:503-26.

Ultrafast endocytosis at mouse hippocampal synapses.
Watanabe S, Rost BR, Camacho-Pérez M, Davis MW, Söhl-Kielczynski B, Rosenmund C, Jorgensen EM.
Nature. 2013 Dec 12;504(7479):242-7.

Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, Rosenmund C.
J Neurosci. 2013 Oct 16;33(42):16698-714.

Endocytosis gets in tune with action potential bursts.
Herman MA, Rosenmund C.
Elife. 2013;2:e01234. doi: 10.7554/eLife.01234.

RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission.
Schwechter B, Rosenmund C, Tolias KF.
Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14462-7.

Syntaxin-1 N-peptide and Habc-domain perform distinct essential functions in synaptic vesicle fusion.
Zhou P, Pang ZP, Yang X, Zhang Y, Rosenmund C, Bacaj T, Südhof TC.
EMBO J. 2013 Jan 9;32(1):159-71.

Activation of metabotropic GABA receptors increases the energy barrier for vesicle fusion.
Rost BR, Nicholson P, Ahnert-Hilger G, Rummel A, Rosenmund C, Breustedt J, Schmitz D.
J Cell Sci. 2011 Sep 15;124(Pt 18):3066-73.

Interplay between VGLUT isoforms and endophilin A1 regulates neurotransmitter release and short-term plasticity.
Weston MC, Nehring RB, Wojcik SM, Rosenmund C.
Neuron. 2011 Mar 24;69(6):1147-59

Dysfunction in GABA signalling mediates autism-like stereotypies and Rett syndrome phenotypes.
Chao HT, Chen H, Samaco RC, Xue M, Chahrour M, Yoo J, Neul JL, Gong S, Lu HC, Heintz N, Ekker M, Rubenstein JL, Noebels JL, Rosenmund C, Zoghbi HY. 
Nature. 2010 Nov 11;468(7321):263-9.

Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7.
Xue M, Craig TK, Shin OH, Li L, Brautigam CA, Tomchick DR, Südhof TC, Rosenmund C, Rizo J. 
PLoS One. 2010 Sep 2;5(9). pii: e12544.

Binding of the complexin N terminus to the SNARE complex potentiates synaptic-vesicle fusogenicity.
Xue M, Craig TK, Xu J, Chao HT, Rizo J, Rosenmund C. 
Nat Struct Mol Biol. 2010 May;17(5):568-75.

Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis.
Shin OH, Lu J, Rhee JS, Tomchick DR, Pang ZP, Wojcik SM, Camacho-Perez M, Brose N, Machius M, Rizo J, Rosenmund C, Südhof TC. 
Nat Struct Mol Biol. 2010 Mar;17(3):280-8.

Tilting the balance between facilitatory and inhibitory functions of mammalian and Drosophila Complexins orchestrates synaptic vesicle exocytosis.
Xue M, Lin YQ, Pan H, Reim K, Deng H, Bellen HJ, Rosenmund C.
Neuron. 2009 Nov 12;64(3):367-80. (Highlighted in F1000 as exceptional)

The headache of a hyperactive calcium channel.
Xue M, Rosenmund C.
Neuron. 2009 Mar 12;61(5):653-4.

Synaptic vesicle fusion.
Rizo J, Rosenmund C.
Nat Struct Mol Biol. 2008 Jul;15(7):665-74.