Rosenmund Lab

In the Rosenmund Lab, we study the process of communication between neurons at their point of contact—the synapse. At the synapse, an incoming electrical response triggers the release of a chemical signal, the neurotransmitter, in a process called exocytosis. The neurotransmitter molecules activate an electrical response in the receiving cell and, thus, the signal is propagated. While many of the essential protein players in transmission at a chemical synapse have been defined, many open questions about the details of this process remain.  

Our overarching goal in the Rosenmund Lab is to understand how different proteins, structures or genes determine neurotransmitter release properties. Why does synaptic transmission differ between cell types? How do proteins work together to assure the speed and efficiency of synaptic exocytosis? We use diverse approaches to reveal the molecular mechanisms underlying synaptic processes

Research Focus

Neurons in the brain transmit information to each other through specialized connections called synapses. This process is initiated when the action potential invades the presynaptic terminal, which in turn causes the fusion of transmitter-filled vesicles with the presynaptic membrane releasing its content. Then transmitters can diffuse through the synaptic cleft and activate postsynaptic receptors thereby altering the postsynaptic membrane potential. This process is highly complex yet occurs with amazing speed and astonishing precision millions of times at every second within our brain. Moreover, functional properties of synapses within the brain can vary dramatically and can undergo rapid and lasting changes, and this in turn affect how information in the brain are encoded, and even how we learn and forget, how we think and feel, how we sense our environment and act. In our lab we study the basic principles of synaptic transmission with a major focus on the process of neurotransmitter release. In particular, we examine the molecular mechanisms underlying this process in central synapses. Within the presynaptic terminal, release of neurotransmitter-filled vesicles is restricted to active zones. In a series of functional highly coordinated and regulated steps synaptic vesicles are filled with neurotransmitter, tether to specific release sites at the active zone, prime to reach fusion competence, and finally fuse in a calcium-triggered event with the plasma membrane to release the neurotransmitter into the synaptic cleft. This chemical signal is than recognized and transduced into an electrical signal by activation of specific receptors at the postsynaptic site. Elucidation of the mechanisms of synaptic transmission and its regulation is central to the understanding of brain function and dysfunction. Our goal is to quantify and kinetically resolve individual steps within this vesicle cycle. We want to understand which presynaptic proteins; protein-domains and individual residues mediate these steps. Furthermore, we want to identify molecules and mechanisms contributing to heterogeneity in synaptic function. Finally, we want to know how changes of synaptic function such as release probability or short-term plasticity affects the behavior of neurons within defined neuronal networks.

Open positions

Currently no open positions.

We always welcome applications for master thesis and PhD collaborations. Please send your application to Christian Rosenmund. Your application should contain a CV and a letter of motivation, including the topic and the method(s) that you are interested in.
Contact:
Prof. Dr. Christian Rosenmund
christian.rosenmund(at)charite.de
+49 30 450 639 090

Lab rotations

You want to do a lab rotation or an internship in our lab? Please send your application to Marion Weber-Boyvat.

Your application should contain a CV and a short letter of motivation, including the topic and the method(s) that you are interested in. Please understand that we cannot accept all applications due to the limitations of our lab ressources.
Contact:
Marion Weber-Boyvat, PhD
marion.weber-boyvat(at)charite.de
+49 30 450 639 090
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Team

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Prof. Christian Rosenmund

Contact Details

Principal Investigator
Curriculum Vitae

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 061
christian.rosenmund(at)charite.de

Image

Heidi Pretorius

Contact Details

Coordinator/Office - AG Rosenmund

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-539 145
heidi.pretorius(at)charite.de

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Melissa Herman, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 174
melissa.herman(at)charite.de

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Dr. Thorsten Trimbuch

Contact Details

Post-Doc
Head of VCF

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 085
thorsten.trimbuch(at)charite.de

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Marion Weber-Boyvat, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 090
Marion.Weber-Boyvat(at)charite.de

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Gülçin Vadar, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 113
guelcin.vardar(at)charite.de

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Estelle Toulmé, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 174
Estelle.Toulme(at)charite.de

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Dr. Marisa Brockmann

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 157
Marisa.Brockmann(at)charite.de

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Marcial Camacho-Perez, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 073
Marcial.Camacho-Perez(at)charite.de

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Beatriz Rebollo González, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 090
beatriz.rebollo-gonzalez(at)charite.de

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Dr. Jana Kroll

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 090
Jana.Kroll(at)charite.de

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Anisha Dayaram, PhD

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-560 169
Anisha.Dayaram(at)charite.de

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Pascal Fenske, MSc.

Contact Details

Post-Doc

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 095
Pascal.Fenske(at)charite.de

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Fereshteh Zarebidaki, MSc.

Contact Details

PhD Student

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 156
Fereshteh.Zarebidaki(at)charite.de

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Boris Bouazza Arostegui, MSc.

Contact Details

PhD Student

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 095
Boris.Bouazza(at)charite.de

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Andrea Salazar Lázaro, MSc.

Contact Details

PhD Student

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 156
Andrea.Salazar-Lazaro(at)charite.de

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Sina Zobel, MSc.

Contact Details

PhD Student

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 156
Sina.Zobel(at)charite.de

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Anne Hahmann, TA

Contact Details

TA

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 156
Anne.Hahmann(at)charite.de

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Berit Söhl-Kielczynski, TA

Contact Details

TA

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 156
Berit.Soehl-Kielczynski(at)charite.de

Image

Heike Lerch, TA

Contact Details

TA

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 073
Heike.Lerch(at)charite.de

Image

Bettina Brokowski, TA

Contact Details

TA

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 113
Bettina.Brokowski(at)charite.de

Image

Katja Pötschke, TA

Contact Details

TA

CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charité Universitaetsmedizin Berlin
Charitéplatz 1
10117 Berlin
Germany

Tel. +49-(0)30-450-639 073
Katja.Poetschke(at)charite.de

Research

Methods

To understand the complexity of synaptic transmission and neurotransmitter release an integrative approach including biochemistry, genetics, structural and functional analysis is required. We functionally characterize synaptic properties of genetic modified mice or human induced neurons that bear deletions of- or carry mutations within presynaptic proteins. By using cultured primary neurons and slices as well as human induced neurons derived from induced pluripotent stem cells we are able to apply the following methods: We use electrical and optical recording techniques, such as standard patch-clamp electrophysiology and Calcium-imaging, to study synapses in their function and plasticity. We further use light and electron microscopy to study the structure of synapses and how these structures change during plastic events and under pathophysiological conditions.
These techniques are also powerful tools in studying the mechanisms that underly diseases such as epilepsy, autism, schizophrenia and many other neurological disorders. Dramatic advances in the field of human and molecular genetics have shown that these diseases are in part synaptopathic, as these diseases are often associated with mutation in synaptic proteins.

Protein structure-function

Thanks to pioneering work in the late 20th century, we know the identities of most proteins involved in neurotransmitter release. However, many details about the structure of these proteins, their interaction partners, and the amino acids essential for their functions are not yet elucidated. In the Rosenmund Lab, we investigate the detailed interactions and mechanisms by which synaptic proteins control neurotransmitter release.
Our approach to investigate the function of synaptic proteins is to perform ‘rescue’ experiments with mutant proteins in neurons where the protein-of-interest has been eliminated. We then assay the effects on neurotransmitter release by assaying the electrophysiological responses of cultured neurons. Our ability to carefully quantify the neurotransmitter release characteristics under different conditions is attributable to our favorite specialized culture system—the autapse.

Transcriptional programs

What are the transcriptional programs that determine synaptic output? How are developmental gene programs influenced by activity? Recent advances in the field of molecular biology allow us to address these questions using RNA sequencing technology. In the Rosenmund Lab, we use a variety of RNA sequencing approaches to investigate the relationship between transcription and synaptic properties.

Functional anatomy

From Katz’s Quantal Hypothesis to Heusser & Reese’s pictures of membrane bound vesicles, much of our knowledge about synaptic transmission has been gained through analysis of the electrical signals and static images of ultrastructure. In the Rosenmund Lab, we try to close the gap between these two techniques, by performing functional anatomy experiments on mammalian central synapses.
We use a cutting edge high-pressure freezing cryo-fixation technique paired with stimulation to investigate the ultrastructure of synapses a specific intervals after neurotransmitter release occurred. Visualizing these processes with electron microscopy has led to a number of exciting discoveries.

Human neuroscience

Ultimately, a major goal in neuroscience research is to understand the human brain, in health and disease. While model systems play an invaluable role in this endeavor, new research has revealed that human neurons have physiological properties that diverge from those of model systems. Differences in function between human neurons and other model organisms is particularly important in modeling disease mechanisms. In the Rosenmund Lab, we investigate aspects of human neurophysiology using human induced pluripotent stem cell (hIPSC) and patient tissue.

Publications

Synaptotagmin-1 drives synchronous Ca2+-triggered fusion by C2B-domain-mediated synaptic-vesicle-membrane attachment.
Chang S, Trimbuch T, Rosenmund C.
Nat Neurosci. 2018 Jan;21(1):33-40. doi: 10.1038/s41593-017-0037-5.


Optogenetic acidification of synaptic vesicles and lysosomes.

Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C.
Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.


Ultrafast endocytosis at mouse hippocampal synapses.

Watanabe S, Rost BR, Camacho-Pérez M, Davis MW, Söhl-Kielczynski B, Rosenmund C, Jorgensen EM.
Nature. 2013 Dec 12;504(7479):242-7.


Interplay between VGLUT isoforms and endophilin A1 regulates neurotransmitter release and short-term plasticity.
Weston MC, Nehring RB, Wojcik SM, Rosenmund C.
Neuron. 2011 Mar 24;69(6):1147-59


Tilting the balance between facilitatory and inhibitory functions of mammalian and Drosophila Complexins orchestrates synaptic vesicle exocytosis.
Xue M, Lin YQ, Pan H, Reim K, Deng H, Bellen HJ, Rosenmund C.
Neuron. 2009 Nov 12;64(3):367-80.
(Highlighted in F1000 as exceptional)


The tetrameric structure of a glutamate receptor channel.
Rosenmund C, Stern-Bach, Y, & Stevens, C. F.
Science. 1998 Jun 5;280(5369):1596-9. doi: 10.1126/science.280.5369.1596..


Definition of the readily releasable pool of vesicles at hippocampal synapses
Rosenmund C, Stevens, C. F.
Neuron. 1996 Jun;16(6):1197-207. doi: 10.1016/s0896-6273(00)80146-4.


Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
J Neurosci. 2020 Dec 2;40(49):9372-9385. doi: 10.1523/JNEUROSCI.1922-20.2020.


SynaptoPAC, an Optogenetic Tool for Induction of Presynaptic Plasticity.
Oldani S, Moreno-Velasquez L, Faiss L, Stumpf A, Rosenmund C, Schmitz D, Rost BR.
J Neurochem. 2020 Oct 9. doi: 10.1111/jnc.15210.


VGLUT2 EXPRESSION IN DOPAMINE NEURONS CONTRIBUTES TO POST-LESIONAL STRIATAL REINNERVATION.
Kouwenhoven WM, Fortin G, Penttinen AM, Florence C, Delignat-Lavaud B, Bourque MJ, Trimbuch T, Luppi MP, Salvail-Lacoste A, Legault P, Poulin JF, Rosenmund C, Awatramani R, Trudeau LÉ
J Neurosci. 2020 Sep 14:JN-RM-0823-20. doi: 10.1523/JNEUROSCI.0823-20.2020.


A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release.
Brockmann MM, Zarebidaki F, Camacho M, Grauel MK, Trimbuch T, Südhof TC, Rosenmund C.
Cell Rep. 2020 Aug 4;32(5):107960. doi: 10.1016/j.celrep.2020.107960.


ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs.
Weber-Boyvat M, Trimbuch T, Shah S, Jäntti J, Olkkonen VM, Rosenmund C.
Cell Mol Life Sci. 2020 Jul 30. doi: 10.1007/s00018-020-03604-w.


Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25.
Malsam J, Bärfuss S, Trimbuch T, Zarebidaki F, Sonnen AF, Wild K, Scheutzow A, Rohland L, Mayer MP, Sinning I, Briggs JAG, Rosenmund C, Söllner TH.
Cell Rep. 2020 Jul 21;32(3):107926. doi: 10.1016/j.celrep.2020.107926.


Epilepsy-causing STX1B mutations translate altered protein functions into distinct phenotypes in mouse neurons.
Vardar G, Gerth F, Schmitt XJ, Rautenstrauch P, Trimbuch T, Schubert J, Lerche H, Rosenmund C, Freund C.
Brain. 2020 Jul 1;143(7):2119-2138. doi: 10.1093/brain/awaa151.


Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice.
Hoffmann-Conaway S, Brockmann MM, Schneider K, Annamneedi A, Rahman KA, Bruns C, Textoris-Taube K, Trimbuch T, Smalla KH, Rosenmund C, Gundelfinger ED, Garner CC, Montenegro-Venegas C.
Elife. 2020 May 4;9:e56590. doi: 10.7554/eLife.56590.


Layer 6b Is Driven by Intracortical Long-Range Projection Neurons.
Zolnik TA, Ledderose J, Toumazou M, Trimbuch T, Oram T, Rosenmund C, Eickholt BJ, Sachdev RNS, Larkum ME.
Cell Rep. 2020 Mar 10;30(10):3492-3505.e5. doi: 10.1016/j.celrep.2020.02.044.


CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A.
Cell Rep. 2020 Feb 18;30(7):2444-2459.e7. doi: 10.1016/j.celrep.2020.01.079.


Calcium-Independent Exo-endocytosis Coupling at Small Central Synapses.
Orlando M, Schmitz D, Rosenmund C, Herman MA.
Cell Rep. 2019 Dec 17;29(12):3767-3774.e3. doi: 10.1016/j.celrep.2019.11.060.


Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses.
Patzke C, Brockmann MM, Dai J, Gan KJ, Grauel MK, Fenske P, Liu Y, Acuna C, Rosenmund C, Südhof TC.
Cell. 2019 Oct 3;179(2):498-513.e22. doi: 10.1016/j.cell.2019.09.011.


The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches.
Brosig A, Fuchs J, Ipek F, Kroon C, Schrötter S, Vadhvani M, Polyzou A, Ledderose J, van Diepen M, Holzhütter HG, Trimbuch T, Gimber N, Schmoranzer J, Lieberam I, Rosenmund C, Spahn C, Scheerer P, Szczepek M, Leondaritis G, Eickholt BJ.
Cell Rep. 2019 Nov 12;29(7):2028-2040.e8. doi: 10.1016/j.celrep.2019.10.039.


LSP5-2157 a new inhibitor of vesicular glutamate transporters.
Poirel O, Mamer LE, Herman MA, Arnulf-Kempcke M, Kervern M, Potier B, Miot S, Wang J, Favre-Besse FC, Brabet I, Laras Y, Bertrand HO, Acher F, Pin JP, Puel JL, Giros B, Epelbaum J, Rosenmund C, Dutar P, Daumas S, El Mestikawy S, Pietrancosta N.
Neuropharmacology. 2020 Mar 1;164:107902. doi: 10.1016/j.neuropharm.2019.107902. Epub 2019 Dec 4.


RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, Sigrist SJ, Rosenmund C, Schmitz D.
Elife. 2019 Sep 19;8. pii: e43243. doi: 10.7554/eLife.43243.


Altered inhibition and excitation in neocortical circuits in congenital microcephaly.
Zaqout S, Blaesius K, Wu YJ, Ott S, Kraemer N, Becker LL, Rosário M, Rosenmund C, Strauss U, Kaindl AM.
Neurobiol Dis. 2019 May 15. pii: S0969-9961(19)30122-6. doi: 10.1016/j.nbd.2019.05.008.


Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC.
Elife. 2019 May 10;8. pii: e46629. doi: 10.7554/eLife.46629.


Glutamatergic innervation onto striatal neurons potentiates GABAergic synaptic output.
Paraskevopoulou F, Herman MA, Rosenmund C
J Neurosci. 2019 Apr 1. pii: 2630-18. doi: 10.1523/JNEUROSCI.2630-18.2019.


Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Sci Rep. 2019 Mar 20;9(1):4890. doi: 10.1038/s41598-019-41259-1.


Membrane bridging by Munc13-1 is crucial for neurotransmitter release.
Quade B, Camacho M, Zhao X, Orlando M, Trimbuch T, Xu J, Li W, Nicastro D, Rosenmund C, Rizo J
Elife. 2019 Feb 28;8. pii: e42806. doi: 10.7554/eLife.42806.


Light-activated ROS production induces synaptic autophagy.
Hoffmann S, Orlando M, Andrzejak E, Bruns C, Trimbuch T, Rosenmund C, Garner CC, Ackermann F
J Neurosci. 2019 Jan 17. pii: 1317-18. doi: 10.1523/JNEUROSCI.1317-18.2019.


Differential pH Dynamics in Synaptic Vesicles From Intact Glutamatergic and GABAergic Synapses.
Herman MA, Trimbuch T, Christian Rosenmund
Front Synaptic Neurosci. 2018 Dec 3;10:44. doi: 10.3389/fnsyn.2018.00044. eCollection 2018.


Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis
Shigeki Watanabe, Lauren Elizabeth Mamer, Sumana Raychaudhuri, Delgermaa Luvsanjav, Julia Eisen, Thorsten Trimbuch, Berit Söhl-Kielczynski, Pascal Fenske, Ira Milosevic, Christian Rosenmund, Erik M. Jorgensen
Neuron 98, 1184–1197, June 27, 2018, doi: 10.1016/j.neuron.2018.06.005.


Synaptotagmin-1 drives synchronous Ca2+-triggered fusion by C2B-domain-mediated synaptic-vesicle-membrane attachment.
Chang S, Trimbuch T, Rosenmund C.
Nat Neurosci. 2018 Jan;21(1):33-40. doi: 10.1038/s41593-017-0037-5.


Cooperative binding mitigates the high-dose hook effect.
Roy RD, Rosenmund C, Stefan MI.
BMC Syst Biol. 2017 Aug 14;11(1):74. doi: 10.1186/s12918-017-0447-8.


Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.
Piwecka M, Glažar P, Hernandez-Miranda LR, Memczak S, Wolf SA, Rybak-Wolf A, Filipchyk A, Klironomos F, Cerda Jara CA, Fenske P, Trimbuch T, Zywitza V, Plass M, Schreyer L, Ayoub S, Kocks C, Kühn R, Rosenmund C, Birchmeier C, Rajewsky N.
Science. 2017 Aug 10. pii: eaam8526. doi: 10.1126/science.aam8526.


Heterodimerization of Munc13 C2A domain with RIM regulates synaptic vesicle docking and priming.
Camacho M, Basu J, Trimbuch T, Chang S, Pulido-Lozano C, Chang SS, Duluvova I, Abo-Rady M, Rizo J, Rosenmund C.
Nat Commun. 2017 May 10;8:15293. doi: 10.1038/ncomms15293.


Characterization of a Human Point Mutation of VGLUT3 (p.A211V) in the Rodent Brain Suggests a Nonuniform Distribution of the Transporter in Synaptic Vesicles.
Ramet L, Zimmermann J, Bersot T, Poirel O, De Gois S, Silm K, Sakae DY, Mansouri-Guilani N, Bourque MJ, Trudeau LE, Pietrancosta N, Daumas S, Bernard V, Rosenmund C, El Mestikawy S.
J Neurosci. 2017 Apr 12;37(15):4181-4199. doi: 10.1523/JNEUROSCI.0282-16.2017. Epub 2017 Mar 17.


ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1143-1161. doi: 10.1083/jcb.201606086. Epub 2017 Mar 6. Erratum in: J Cell Biol. 2017


Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1C2BMUN.
Xu J, Camacho M, Xu Y, Esser V, Liu X, Trimbuch T, Pan YZ, Ma C, Tomchick DR, Rosenmund C, Rizo J.
Elife. 2017 Feb 8;6. pii: e22567. doi: 10.7554/eLife.22567.


Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons.
Sampathkumar C, Wu YJ, Vadhvani M, Trimbuch T, Eickholt B, Rosenmund C.
Elife. 2016 Oct 26;5. pii: e19374. doi: 10.7554/eLife.19374.


RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, Rost BR, Eickholt BJ, Haucke V, Schmitz D, Sigrist SJ, Rosenmund C.
Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11615-11620. Epub 2016 Sep 26.


Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons.
Vardar G, Chang S, Arancillo M, Wu YJ, Trimbuch T, Rosenmund C.
J Neurosci. 2016 Jul 27;36(30):7911-24. doi: 10.1523


Functional Synergy between the Munc13 C-terminal C1 and C2 domains.
Liu X, Seven AB, Camacho M, Esser V, Xu J, Trimbuch T, Quade B, Su L, Ma C, Rosenmund C, Rizo J.
eLife 2016;10.7554/eLife.13696


Should I stop or should I go? The role of complexin in neurotransmitter release.
Trimbuch T, Rosenmund C.
Nature Reviews Neuroscience  17, 118–125 (2016)


Optogenetic acidification of synaptic vesicles and lysosomes.
Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C.
Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.


Co-release of glutamate and GABA from single vesicles in GABAergic neurons exogenously expressing VGLUT3.
Zimmermann J, Herman MA, Rosenmund C.
Front Synaptic Neurosci. 2015 Sep 23;7:16


Syntaxin 1B is important for mouse postnatal survival and proper synaptic function at the mouse neuromuscular junctions.
Wu Y-J, Tejero R, Arancillo M, Vardar G, Korotkova T, Kintscher M, Schmitz D, Ponomarenko A, Tabares L, Rosenmund C.
Journal of Neurophysiology Published 1 October 2015 Vol. 114 no. 4, 2404-2417 DOI: 10.1152/jn.00577.2015


Ligand-dependent opening of the multiple AMPA receptor conductance states: a concerted model.
Dutta-Roy R, Rosenmund C, Edelstein SJ, Le Novère N.
PLoS One. 2015 Jan 28;10(1):e0116616. doi: 10.1371/journal.pone.0116616.


On the Brink: A New Synaptic Vesicle Release Model at the Calyx of Held.
Herman MA, Rosenmund C.
Neuron. 2015 Jan 7;85(1):6–8


Clathrin regenerates synaptic vesicles from endosomes.
Watanabe S, Trimbuch T, Camacho-Pérez M, Rost BR, Brokowski B, Söhl-Kielczynski B, Felies A, Davis MW, Rosenmund C, Jorgensen EM.
Nature. 2014 Nov 13;515(7526):228-33


The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones.
Imig C, Min SW, Krinner S, Arancillo M, Rosenmund C, Südhof TC, Rhee J, Brose N, Cooper BH.
Neuron. 2014 Oct 22;84(2):416-31


Synaptobrevin 1 mediates vesicle priming and evoked release in a subpopulation of hippocampal neurons.
Zimmermann J, Trimbuch T, Rosenmund C.
J Neurophysiol. 2014 Sep 15;112(6):1559-65.


Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture.
Herman MA, Ackermann F, Trimbuch T, Rosenmund C.
J Neurosci. 2014 Aug 27;34(35):11781-91.


Biophysical properties of presynaptic short-term plasticity in hippocampal neurons: insights from electrophysiology, imaging and mechanistic models.
Dutta Roy R, Stefan MI, Rosenmund C.
Front Cell Neurosci. 2014 May 22;8:141


Re-examining how complexin inhibits neurotransmitter release: SNARE complex insertion or electrostatic hindrance?
Trimbuch T, Xu J, Flaherty D, Tomchick DR, Rizo J, Rosenmund C.
eLife 2014;10.7554/eLife.02391


Biophysical properties of presynaptic short-term plasticity in hippocampal neurons: insights from electrophysiology, imaging and mechanistic models.
Dutta Roy R, Stefan MI, and Rosenmund C.
Front. Cell. Neurosci. | doi: 10.3389/fncel.2014.00141 


Investigation of Synapse Formation and Function in a Glutamatergic-GABAergic Two-Neuron Microcircuit.
Chang CL, Trimbuch T, Chao HT, Jordan JC, Herman MA, Rosenmund C.
J Neurosci. 2014 Jan 15;34(3):855-68.


Nanometer-resolution fluorescence electron microscopy (nano-EM) in cultured cells.
Watanabe S, Lehmann M, Hujber E, Fetter RD, Richards J, Söhl-Kielczynski B, Felies A, Rosenmund C, Schmoranzer J, Jorgensen EM.
Methods Mol Biol. 2014;1117:503-26.


Ultrafast endocytosis at mouse hippocampal synapses.
Watanabe S, Rost BR, Camacho-Pérez M, Davis MW, Söhl-Kielczynski B, Rosenmund C, Jorgensen EM.
Nature. 2013 Dec 12;504(7479):242-7.


Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, Rosenmund C.
J Neurosci. 2013 Oct 16;33(42):16698-714.


Endocytosis gets in tune with action potential bursts.
Herman MA, Rosenmund C.
Elife. 2013;2:e01234. doi: 10.7554/eLife.01234.


RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission.
Schwechter B, Rosenmund C, Tolias KF.
Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14462-7.


Syntaxin-1 N-peptide and Habc-domain perform distinct essential functions in synaptic vesicle fusion.
Zhou P, Pang ZP, Yang X, Zhang Y, Rosenmund C, Bacaj T, Südhof TC.
EMBO J. 2013 Jan 9;32(1):159-71.


Activation of metabotropic GABA receptors increases the energy barrier for vesicle fusion.
Rost BR, Nicholson P, Ahnert-Hilger G, Rummel A, Rosenmund C, Breustedt J, Schmitz D.
J Cell Sci. 2011 Sep 15;124(Pt 18):3066-73.


Interplay between VGLUT isoforms and endophilin A1 regulates neurotransmitter release and short-term plasticity.
Weston MC, Nehring RB, Wojcik SM, Rosenmund C.
Neuron. 2011 Mar 24;69(6):1147-59


Dysfunction in GABA signalling mediates autism-like stereotypies and Rett syndrome phenotypes.
Chao HT, Chen H, Samaco RC, Xue M, Chahrour M, Yoo J, Neul JL, Gong S, Lu HC, Heintz N, Ekker M, Rubenstein JL, Noebels JL, Rosenmund C, Zoghbi HY. 
Nature. 2010 Nov 11;468(7321):263-9.


Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7.
Xue M, Craig TK, Shin OH, Li L, Brautigam CA, Tomchick DR, Südhof TC, Rosenmund C, Rizo J. 
PLoS One. 2010 Sep 2;5(9). pii: e12544.


Binding of the complexin N terminus to the SNARE complex potentiates synaptic-vesicle fusogenicity.
Xue M, Craig TK, Xu J, Chao HT, Rizo J, Rosenmund C. 
Nat Struct Mol Biol. 2010 May;17(5):568-75.


Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis.
Shin OH, Lu J, Rhee JS, Tomchick DR, Pang ZP, Wojcik SM, Camacho-Perez M, Brose N, Machius M, Rizo J, Rosenmund C, Südhof TC. 
Nat Struct Mol Biol. 2010 Mar;17(3):280-8.


Tilting the balance between facilitatory and inhibitory functions of mammalian and Drosophila Complexins orchestrates synaptic vesicle exocytosis.
Xue M, Lin YQ, Pan H, Reim K, Deng H, Bellen HJ, Rosenmund C.
Neuron. 2009 Nov 12;64(3):367-80.
(Highlighted in F1000 as exceptional)


The headache of a hyperactive calcium channel.
Xue M, Rosenmund C.
Neuron. 2009 Mar 12;61(5):653-4.


Synaptic vesicle fusion.
Rizo J, Rosenmund C.
Nat Struct Mol Biol. 2008 Jul;15(7):665-74.

Contact

Rosenmund Lab
Charité - Universitätsmedizin Berlin
CC02 für Grundlagenmedizin
Neurowiss. für zelluläre molek. Neurobiologie
-AG Rosenmund-
Charitéplatz 1
10117 Berlin
Germany

info(at)rosenmundlab.de

Our lab is located in the Charite-Cross-Over (CCO) Building at the Charité Campus Mitte in Berlin.

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